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Subject    Construction of efficient xylose-fermenting Saccharomyces cerevisiae through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis
Jung-Hyun Jo, Jin-Ho Seo et al.
Bioresource Technology
Construction of efficient xylose-fermenting Saccharomyces cerevisiae through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis 

Jung-Hyun Jo, Yong-Cheol Park, Yong-Su Jin and Jin-Ho Seo
Bioresource Technology
241(2017) 88-94


Abstract
Engineered Saccharomyces cerevisiae has been used for ethanol production from xylose, the abundant sugar in lignocellulosic hydrolyzates. Development of engineered S. cerevisiae able to utilize xylose effectively is crucial for economical and sustainable production of fuels. To this end, the xylose-metabolic genes (XYL1, XYL2 and XYL3) from Scheffersomyces stipitis have been introduced into S. cerevisiae. The resulting engineered S. cerevisiae strains, however, often exhibit undesirable phenotypes such as slow xylose assimilation and xylitol accumulation. This work was undertaken to construct an improved xylose-fermenting strain by developing a synthetic isozyme system of xylose reductase (XR). The DXS strain having both wild XR and mutant XR showed low xylitol accumulation and fast xylose consumption compared to the engineered strains expressing only one type of XRs, resulting in improved ethanol yield and productivity. These results suggest that the introduction of the XR-based synthetic isozyme system is a promising strategy to develop efficient xylose-fermenting strains.

KEYWORDS : Saccharomyces cerevisiae, Xylose, Cellulosic ethanol, Synthetic isozyme system, Xylose reductase




No
Category
Subject
Name
Date
Hit
202 2017     Enhanced production of 2,3-butanediol from xylose by combinatorial engineering of xylose metabolic pathway and cofactor regeneration in pyruvate decarboxylase-deficient Saccharomyces cerevisiae
Soo-Jung Kim,Hee-Jin Sim,Jin-Ho Seo
2017/06/07  267
2017     Construction of efficient xylose-fermenting Saccharomyces cerevisiae through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis
Jung-Hyun Jo, Jin-Ho Seo et al.
Bioresource Technology
2017/05/25  268
200 2017     Metabolic engineering of Saccharomyces cerevisiae for production of spermidine under optimal culture conditions
Sun-Ki Kim, Jung-Hyun Jo, Yong-Cheol Park, Yong-Su Jin, Jin-Ho Seo et al.
Enzyme and Microbial Technology
2017/03/22  675
199 2017     High production of 2,3-butanediol from glycerol without 1,3-propanediol formation by Raoultella ornithinolytica B6
Taeyeon Kim, Jin-Ho Seo et al.
Applied Microbiology and Biotechnology
2017/03/20  498
198 2016     Metabolic engineering of Saccharomyces cerevisiae for 2,3-butanediol production
Soo-Jung Kim, Jin-Ho Seo. et al.
Applied Microbiology and Biotechnology
2017/02/03  845
197 2016     Enhanced ethanol fermentation by engineered Saccharomyces cerevisiae strains with high spermidine contents
Sun-Ki Kim, Jung-Hyun Jo, Yong-Su Jin, and Jin-Ho Seo. et al.
Bioprocess and Biosystems Engineering
2016/12/27  861
196 2016     Bioethanol production from cellulosic hydrolysates by engineered industrial Saccharomyces cerevisiae
Ye-Ji Lee , Yong-Su Jin, Jin-Ho Seo. et al.
Bioresource Technology
2016/12/12  875
195 2016     Improved production of 2’-fucosyllactose in engineered Escherichia coli by expressing putative α-1,2-fucosyltransferase, WcfB from Bacteroides fragilis
Young-Wook Chin, Jin-Ho Seo. et al.
Journal of Biotechnolog
2016/12/01  802
194 2016     Enhanced production of 2,3-butanediol by engineered Saccharomyces cerevisiae through fine-tuning of pyruvate decarboxylase and NADH oxidase activities.
Jin-Woo Kim, Jin-Ho Seo. et al.
Biotechnology for Biofuels
2016/11/25  711
193 2016     High Production of 2,3-Butanediol (2,3-BD) by Raoultella ornithinolytica B6 via Optimizing Fermentation Conditions and Overexpressing 2,3-BD Synthesis Genes.
Tae-yeon Kim, Jin-Ho Seo et al.
PLoS ONE
2016/10/25  719
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