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Subject    Enhanced production of 2,3-butanediol from xylose by combinatorial engineering of xylose metabolic pathway and cofactor regeneration in pyruvate decarboxylase-deficient Saccharomyces cerevisiae
Soo-Jung Kim,Hee-Jin Sim,Jin-Ho Seo
Enhanced production of 2,3-butanediol from xylose by combinatorial engineering of xylose metabolic pathway and cofactor regeneration in pyruvate decarboxylase-deficient Saccharomyces cerevisiae 

Soo-Jung Kim Hee-Jin Sim, Jin-Woo Kim, Ye-Gi Lee, Yong-Cheol Park and Jin-Ho Seo
Bioresource Technology
Available online 9 June 2017


Abstract
The aim of this study was to produce 2,3-butanediol (2,3-BDO) from xylose efficiently by modulation of the xylose metabolic pathway in engineered Saccharomyces cerevisiae. Expression of the Scheffersomyces stipitis transaldolase and NADH-preferring xylose reductase in S. cerevisiae improved xylose consumption rate by a 2.1-fold and 2,3-BDO productivity by a 1.8-fold. Expression of the Lactococcus lactis noxE gene encoding NADH oxidase also increased 2,3-BDO yield by decreasing glycerol accumulation. Additionally, the disadvantage of C2-dependent growth of pyruvate decarboxylase-deficient (Pdc-) S. cerevisiae was overcome by expression of the Candida tropicalis PDC1 gene. A fed-batch fermentation of the BD5X-TXmNP strain resulted in 96.8 g/L 2,3-BDO and 0.58 g/L-h productivity from xylose, which were 15.6- and 2-fold increases compared with the corresponding values of the BD5X strain. It was concluded that facilitation of the xylose metabolic pathway, oxidation of NADH and relief of C2-dependency synergistically triggered 2,3-BDO production from xylose in Pdc-S. cerevisiae.

KEYWORDS : 2,3-Butanediol; Xylose; noxE; TAL1; Pyruvate decarboxylase-deficient Saccharomyces cerevisiae




No
Category
Subject
Name
Date
Hit
203 2017     One-pot synthesis of GDP-L-fucose by a four-enzyme cascade expressed in Lactococcus lactis
Ling Li, Jin-ho Seo, Nam Soo Han et al.
Journal of Biotechnology
2017/10/16  111
2017     Enhanced production of 2,3-butanediol from xylose by combinatorial engineering of xylose metabolic pathway and cofactor regeneration in pyruvate decarboxylase-deficient Saccharomyces cerevisiae
Soo-Jung Kim,Hee-Jin Sim,Jin-Ho Seo
2017/06/07  506
201 2017     Construction of efficient xylose-fermenting Saccharomyces cerevisiae through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis
Jung-Hyun Jo, Jin-Ho Seo et al.
Bioresource Technology
2017/05/25  429
200 2017     Metabolic engineering of Saccharomyces cerevisiae for production of spermidine under optimal culture conditions
Sun-Ki Kim, Jung-Hyun Jo, Yong-Cheol Park, Yong-Su Jin, Jin-Ho Seo et al.
Enzyme and Microbial Technology
2017/03/22  878
199 2017     High production of 2,3-butanediol from glycerol without 1,3-propanediol formation by Raoultella ornithinolytica B6
Taeyeon Kim, Jin-Ho Seo et al.
Applied Microbiology and Biotechnology
2017/03/20  622
198 2016     Metabolic engineering of Saccharomyces cerevisiae for 2,3-butanediol production
Soo-Jung Kim, Jin-Ho Seo. et al.
Applied Microbiology and Biotechnology
2017/02/03  1048
197 2016     Enhanced ethanol fermentation by engineered Saccharomyces cerevisiae strains with high spermidine contents
Sun-Ki Kim, Jung-Hyun Jo, Yong-Su Jin, and Jin-Ho Seo. et al.
Bioprocess and Biosystems Engineering
2016/12/27  1013
196 2016     Bioethanol production from cellulosic hydrolysates by engineered industrial Saccharomyces cerevisiae
Ye-Ji Lee , Yong-Su Jin, Jin-Ho Seo. et al.
Bioresource Technology
2016/12/12  1030
195 2016     Improved production of 2’-fucosyllactose in engineered Escherichia coli by expressing putative α-1,2-fucosyltransferase, WcfB from Bacteroides fragilis
Young-Wook Chin, Jin-Ho Seo. et al.
Journal of Biotechnolog
2016/12/01  964
194 2016     Enhanced production of 2,3-butanediol by engineered Saccharomyces cerevisiae through fine-tuning of pyruvate decarboxylase and NADH oxidase activities.
Jin-Woo Kim, Jin-Ho Seo. et al.
Biotechnology for Biofuels
2016/11/25  856
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