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Subject    Improved production of 2’-fucosyllactose in engineered Escherichia coli by expressing putative α-1,2-fucosyltransferase, WcfB from Bacteroides fragilis
Young-Wook Chin, Jin-Ho Seo. et al.
Journal of Biotechnolog
Improved production of 2’-fucosyllactose in engineered Escherichia coli by expressing putative α-1,2-fucosyltransferase, WcfB from Bacteroides fragilis.

Young-Wook Chin, Ji-Yeong Kim, Jae-Han Kim, Sang-Min Jung and Jin-Ho Seo.

Journal of Biotechnology ( available online at :
http://dx.doi.org/10.1016/j.jbiotec.2016.11.033 )


ABSTRACT
2’-Fucosyllactose (2-FL) is one of most abundant oligosaccharides in human milk, which is involved in many biological functions for infant health. Since 2-FL has a great potential in application to functional food materials and pharmaceuticals, several microbial systems for mass production of 2-FL have been developed in recent years. Microbial production of 2-FL was suggested to be influenced by a number of factors including fucosylation activity of α-1,2-fucosyltransferase. In the present study, the wcfB gene coding for α-1,2-fucosyltransferase from Bacteroides fragilis was screened from eleven candidates of putative α-1,2-fucosyltransferase. Introduction of the wcfB gene allows the lacZ-deleted strain of E. coli expressing the genes for guanosine 5’-diphosphate (GDP)-L-fucose biosynthetic enzymes to produce 2-FL. As a result of fed-batch fermentation, 15.4 g/L extracellular concentration of 2-FL with 2-FL yield of 0.858 g/g lactose and productivity of 0.530 g/L/h were obtained. In addition, the feasibility of industrial production of 2-FL using this microbial system was demonstrated by performing fed-batch fermentation in a 75 L bioreactor.




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206 2018     Elimination of biosynthetic pathways for L-valine and L-isoleucine in mitochondria enhances isobutanol production in engineered Saccharomyces cerevisiae
Kyung-Muk Lee, Sun-Ki Kim, Ye-Gi Lee, Kyung-Hye Park, Jin-Ho Seo et al.
2018/08/06  87
205 2018     Anti-melanogenic activity of schaftoside in Rhizoma Arisaematis by increasing autophagy in B16F1 cells
Pan Soo Kim, Jin-Ho Seo et al.
Biochemical and Biophysical Research Communications
2018/06/11  191
204 2018     Engineering of α−1,3-fucosyltransferases for production of 3-fucosyllactose in Escherichia coli
Jiwon Yu, Sang-Min Jung, Jin-Ho Seo and Dae-Hyuk Kweon et al.
Metabolic Engineering
2018/06/11  192
203 2017     One-pot synthesis of GDP-L-fucose by a four-enzyme cascade expressed in Lactococcus lactis
Ling Li, Jin-ho Seo, Nam Soo Han et al.
Journal of Biotechnology
2017/10/16  790
202 2017     Enhanced production of 2,3-butanediol from xylose by combinatorial engineering of xylose metabolic pathway and cofactor regeneration in pyruvate decarboxylase-deficient Saccharomyces cerevisiae
Soo-Jung Kim,Hee-Jin Sim,Jin-Ho Seo
2017/06/07  1461
201 2017     Construction of efficient xylose-fermenting Saccharomyces cerevisiae through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis
Jung-Hyun Jo, Jin-Ho Seo et al.
Bioresource Technology
2017/05/25  1173
200 2017     Metabolic engineering of Saccharomyces cerevisiae for production of spermidine under optimal culture conditions
Sun-Ki Kim, Jung-Hyun Jo, Yong-Cheol Park, Yong-Su Jin, Jin-Ho Seo et al.
Enzyme and Microbial Technology
2017/03/22  1601
199 2017     High production of 2,3-butanediol from glycerol without 1,3-propanediol formation by Raoultella ornithinolytica B6
Taeyeon Kim, Jin-Ho Seo et al.
Applied Microbiology and Biotechnology
2017/03/20  1355
198 2016     Metabolic engineering of Saccharomyces cerevisiae for 2,3-butanediol production
Soo-Jung Kim, Jin-Ho Seo. et al.
Applied Microbiology and Biotechnology
2017/02/03  1758
197 2016     Enhanced ethanol fermentation by engineered Saccharomyces cerevisiae strains with high spermidine contents
Sun-Ki Kim, Jung-Hyun Jo, Yong-Su Jin, and Jin-Ho Seo. et al.
Bioprocess and Biosystems Engineering
2016/12/27  1586
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