Biomolecular Engineering Lab.
아이디
비밀번호




Animate

 

Publications

 Total 206articles,
 Now page is 7 / 21pages
View Article     
Name   
Subject   Effects of Trx2p and Sec23p expression on stable production of hepatitis B surface antigen S domain in recombinant Saccharomyces cerevisiae
Young-Kyoung Park et al.
Journal of Biotechnology 2012 160:151-160
Effects of Trx2p and Sec23p expression on stable production of hepatitis B surface antigen S domain in recombinant Saccharomyces cerevisiae

Young-Kyoung Park; Sang-Min Jung; Hyung-Kwon Lim; Young-Jin Son; Yong-Cheol Park; Jin-Ho Seo

Journal of Biotechnology 2012 160:151-160
ABSTRACT: The S domain of hepatitis B virus surface antigen (sHBsAg) is the primary
component for vaccine development against virus infection. For stable expression of
sHBsAg in recombinant Saccharomyces cerevisiae, new accessory genes necessary for
foreign protein expression were screened by DNA microarray. Among 600 genes of
interest, genes coding for an activating protein of ATPase in Hsp90 (Aha1p),
Saccharomyces cerevisiae DnaJ (Scj1p), thioredoxin 2 (Trx2p) and a GTPase-activator
specific for Sar1 (Sec23p) as well as Pdi1p were selected in transcriptome analysis,
which are known to facilitate disulfide bond formation or induce protein transport
in the secretion pathway. Individual and combinatorial expression of SEC23, TRX2
and PDI1 increased total sHBsAg concentration by 1.9-6.5 folds, relative to the control
strain expressing sHBsAg only. Additionally, moderate expression of Kex2p protease
able to cut off the signal peptide enhanced the portion of the authentic sHBsAg to total
sHBsAg. Fed-batch fermentation of the S. cerevisiae 2805 strain coexpressing the sHBsAg,
SEC23, PDI1 and KEX2 genes resulted in 70.6 mg/L final sHBsAg concentration which
was 5.6 times higher than that of the control. Transmission electron microscopic analysis
of the yeast cells elucidated the effects of the accessory gene coexpression on the
intracellular localization of sHBsAg. Like PDI1, coexpression of both SEC23 and/or TRX2
newly isolated in this study is expected to improve the target protein expression
in S. cerevisiae.




No
Category
Subject
Name
Date
Hit
146 2012    Characterization of Saccharomyces cerevisiae promoters for heterologous gene expression in Kluyveromyces marxianus
Dae-Hyuk Kweon et al.
Applied Microbiology and Biotechnology 2013 97 (5):2029–2041
2012/07/16  3208
145 2012    Production of resveratrol from tyrosine in metabolically engineered Saccharomyces cerevisiae
So-Yeon Shin et al.
Enzyme and Microbial Technology 2012 51(4);211–216
2012/06/27  2731
2012    Effects of Trx2p and Sec23p expression on stable production of hepatitis B surface antigen S domain in recombinant Saccharomyces cerevisiae
Young-Kyoung Park et al.
Journal of Biotechnology 2012 160:151-160
2012/05/08  2658
143 2012    Aqueous ammonia pretreatment, saccharification, and fermentation evaluation of oil palm fronds for ethanol production
Kyoung Heon Kim et al.
Bioprocess and Biosystems Engineering 2012 35(9):1497-1503
2012/04/19  2984
142 2012    Whole cell biosynthesis of a functional oligosaccharide, 2-fucosyllactose, using engineered Escherichia coli
Won-Heong Lee et al.
Microbial Cell Factories 2012 11:48
2012/04/18  2926
141 2012    Electrostatic interaction-induced inclusion body formation of glucagon-like peptide-1 fused with ubiquitin and cationic tag
Sung-Gun Kim et al.
Protein Expression and Purification 2012 84(1):38-46
2012/04/17  2658
140 2012    pH-responsive high-density lipoprotein-like nanoparticles release paclitaxel at acidic pH in cancer chemotherapy
Sung-Gun Kim, Dae-Hyuk Kweon et al.
International Journal of Nanomedicine 2012:7 1–12
2012/04/16  2288
139 2012    Isobutanol production in engineered Saccharomyces scerevisiae by overexpression of 2-ketoisovalerate decarboxylase and valine biosynthetic enzymes
Won-Heong Lee et al.
Bioprocess and Biosystems Engineering 2012 35(9
2012/04/10  2195
138 2012    Modulation of guanosine nucleotides biosynthetic pathways enhanced GDP-L-fucose production in recombinant Escherichia coli
Won-Heong Lee et al.
Applied microbiology and biotechnology 2012 93:2327-2334
2011/11/28  2812
137 2011    Biotechnological production of human milk oligosaccharides
Nam Soo Han et al.
Biotechnology Advances 2012 30:1268-1278
2011/11/09  3057
Prev [1][2][3][4][5][6] 7 [8][9][10]..[21] Next
Copyright 1999-2018 Zeroboard / skin by lifesay






The information contained in this web site is subject to change without notice.
Copyright ⓒ 1996-2004Biomolecular Engineering Laboratory. All rights reserved.

Biomolecular Engineering Laboratory, Room 200-BIO 8104
Department of Agricultural Biotechnology, Seoul National University
San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Korea

Tel: +82-2-880-4862 Fax: +82-2-873-5095